ABSTRACT
During the Covid-19 global pandemic, exposure to cold cargo surfaces contaminated with Covid-19 was first identified as a potential cause of infection. Given that the epidemic situation in China is basically stable, epidemic prevention and control of cold chain cargo handling operations in Chinese ports is one of the key points for the country. It is concluded that the main risk link that may cause the spread of the epidemic is the unpacking of cold chain cargo containers by analyzing the process and characteristics of port cold chain cargo handling.In order to prevent imported epidemics from abroad, Chinese ports have taken countermeasures such as virus detection and sterilization. At present, nucleic acid detection measures have been adopted for the virus detection on the surface of goods, but the sampling quantity and method are lack of unified regulations, and the detection takes a long time. The mobile cabin PCR laboratories are used in some areas to improve the timeliness, and the virus detection on the surface of goods needs more sensitive and rapid detection technology. In the process of comprehensive preventive disinfection of goods, it was found that the disinfection efficiency of common disinfectants in low-temperature environment was greatly reduced, and a variety of new low-temperature disinfectants were rapidly developed. The disinfection technology based on deep UV LED, UV catalysis, nuclear radiation and other physical technologies have brought a new revolution to the disinfection of the new coronavirus on the surface of low-temperature objects.Due to the global pandemic of novel coronavirus and its continuous variation, technical measures for epidemic prevention and control have developed rapidly. From the prevention and control experience of Chinese ports in combating the epidemic, epidemic prevention and control is a systematic project, which requires the combination of various technical measures and close cooperation of multiple links. © 2022 SPIE.
ABSTRACT
Rolling Circle Amplification (RCA) has shown significant potential for pathogen diagnostics providing high specificity and sensitivity combined with relatively low temperature (<37 °C) isothermal amplification. In the context of the ongoing COVID-19 pandemic, we report the development of an RCA-based method allowing direct detection of SARS-CoV-2 RNA in microfluidics. The viral RNA was hybridized to biotinylated oligos and L-probes in solution, enriched in a microchannel and subsequently amplified in situ using padlock probes against the L-probes. This method allowed the detection of 1x103 viral copies/μL within 90 minutes of amplification, demonstrating an alternative approach to current isothermal amplification methods. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.